Many times on Workshop Wednesdays typos are pointed out by fellow writers in the comments, and rightfully so: making your query and your manuscript as clean as possible is important. However, recently one commenter pointed out a typo in the first line and asked, "Would a typo in the first line not make you want to reject it?"
There's a distinct difference between a typo and a writer who doesn't have a good grasp of the English language. I'd like to think that a lot of the time I can tell the difference. Sure, typos might hurt your query and you should do your best to eliminate them, but a good query, a great and intriguing story, will rise above all typos.